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DSIP (Delta Sleep-Inducing Peptide)

DSIP (Delta Sleep-Inducing Peptide, CAS 62568-57-4) is an endogenous nonapeptide (Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu) with a molecular weight of approximately 848.8 Da and formula C₃₅H₄₈N₁₀O₁₅. First isolated from the cerebral venous blood of rabbits subjected to electrical thalamic stimulation, DSIP was identified as a humoral sleep-promoting factor. It is a specialized research tool for studying EEG sleep architecture, neuroendocrine regulation, and stress response modulation.

Technical Specifications

Property Value
CAS Number 62568-57-4
Molecular Formula C₃₅H₄₈N₁₀O₁₅
Molecular Weight ~848.8 Da
Purity ≥99% (HPLC-verified)
Appearance Lyophilized white powder
Solubility Soluble in sterile water and aqueous buffers
Storage -20°C (lyophilized), 2–8°C (reconstituted)

Mechanism of Action

DSIP modulates sleep architecture through central neuromodulatory mechanisms involving the serotonergic, GABAergic, and hypothalamic-pituitary-adrenal (HPA) axis systems. EEG studies in multiple species demonstrate that DSIP administration increases slow-wave sleep (delta activity, 0.5–4 Hz) and spindle activity without significantly affecting REM sleep duration. The peptide appears to act through a specific DSIP receptor or binding site in the brain, distinct from classical neurotransmitter receptors, which triggers downstream signaling that potentiates GABAergic inhibitory tone in thalamocortical circuits — the neural substrate of sleep spindle generation and delta wave synchronization. Additionally, DSIP suppresses corticotropin-releasing hormone (CRH) release from the paraventricular nucleus, attenuating ACTH and cortisol secretion and thereby reducing stress-induced sleep disruption. DSIP also modulates the endogenous opioid system, potentiating the effects of beta-endorphin, and has been shown to influence circadian rhythm entrainment through effects on the suprachiasmatic nucleus.

Research Applications

  • Primary: Sleep neurophysiology — EEG sleep architecture, slow-wave sleep mechanisms, delta wave generation
  • Secondary: Stress response and HPA axis modulation, circadian rhythm biology, opioid system interaction studies
  • Model Systems: In vitro (neuronal receptor binding assays, hypothalamic slice electrophysiology), in vivo (EEG/EMG sleep recording in rodents, stress-induced sleep disruption models)

Quality Control & Analytical Methods

  • HPLC: ≥99% purity verification at 214/220 nm (C18 reversed-phase column)
  • Mass Spectrometry: ESI-MS for molecular weight confirmation (~848.8 ±1.0 Da)
  • Peptide Content: Amino acid analysis (AAA) for net peptide content determination
  • Endotoxin: <1 EU/mg (LAL assay)
  • TFA Content: <1% (ion chromatography)

Stability & Storage

Condition Stability
-20°C (lyophilized) 24 months
4°C (lyophilized) 6 months
25°C (lyophilized) 1 month
Reconstituted (4°C) 7 days
Reconstituted (-20°C) 30 days

Key Research References

  • Monnier et al. (1977) — The delta sleep inducing peptide (DSIP). Isolation and characterization. Neurosci Lett. PMID: 19604517
  • Graf & Kastin (1986) — Delta-sleep-inducing peptide (DSIP): an update. Peptides. PMID: 3547332
  • Pollmächer et al. (1997) — Effects of DSIP on sleep and the HPA axis in humans. Neuropsychobiology. PMID: 9307234

Source & Purchase

For researchers requiring research-grade DSIP with full analytical documentation including HPLC, LC-MS, and Certificate of Analysis, visit the HK Peptides product page for specifications, bulk pricing, and ordering.

View DSIP Product →

FAQ

Q: What purity level is standard for DSIP? A: HK Peptides supplies DSIP at ≥99% purity by HPLC with full COA documentation.

Q: How should DSIP be stored for research use? A: Lyophilized DSIP should be stored at -20°C. Reconstituted solutions at 4°C for short-term use (up to 7 days).